The detection of synergy between meropenem and polymyxin B against meropenem-resistant Acinetobacter baumannii using Etest and time-kill assay

George A Pankey; Deborah S Ashcraft

doi:10.1016/j.diagmicrobio.2008.11.002

The detection of synergy between meropenem and polymyxin B against meropenem-resistant Acinetobacter baumannii using Etest and time-kill assay

Diagn Microbiol Infect Dis. 2009 Feb;63(2):228-32. doi: 10.1016/j.diagmicrobio.2008.11.002.

Authors

George A Pankey¹, Deborah S Ashcraft

Affiliation

¹ Infectious Disease Research, Ochsner Clinic Foundation, New Orleans, LA 70121, USA. gpankey@ochsner.org

PMID: 19150711
DOI: 10.1016/j.diagmicrobio.2008.11.002

Abstract

Time-kill assay and Etest testing for synergy of meropenem (MER) (1x MIC) plus polymyxin B (1/4, 1/2, and 1x MIC) were performed against 8 genetically unique MER-resistant clinical Acinetobacter baumannii isolates. Time-kill assay demonstrated synergy for all isolates, whereas Etest showed synergy in 5 isolates and indifference in 3.

MeSH terms

Acinetobacter Infections / microbiology
Acinetobacter baumannii / drug effects
Acinetobacter baumannii / genetics*
Anti-Bacterial Agents / pharmacology*
Clinical Laboratory Techniques
Colony Count, Microbial / methods
Drug Resistance, Bacterial / genetics*
Drug Resistance, Bacterial / physiology
Drug Synergism
Humans
Meropenem
Microbial Sensitivity Tests
Polymyxin B / pharmacology*
Thienamycins / pharmacology*

Substances

Anti-Bacterial Agents
Thienamycins
Meropenem
Polymyxin B