Langmuir-Blodgett film based on MEH-PPV for cholesterol biosensor

Anal Chim Acta. 2009 Feb 23;634(2):243-9. doi: 10.1016/j.aca.2008.12.023. Epub 2008 Dec 24.

Abstract

Cholesterol oxidase (ChOx) has been immobilized onto conducting poly[2-methoxy,5-(2'-ethyl-hexyloxy)-1,4-phenylene vinylene] (MEH-PPV)/stearic acid (SA) Langmuir-Blodgett film transferred onto octadecanethiol (ODT) modified gold plate. The ChOx/MEH-PPV/SA LB film bioelectrode exhibits has been characterized by FT-IR, contact angle, and atomic force microscopy. The response of the ChOx/MEH-PPV/SA LB film bioelectrode carried out using differential pulse voltammetry (DPV) studies reveal linearity from 1.29 to 12.91 mM of cholesterol concentration and response time as 30 s. This ChOx/MEH-PPV/SA bioelectrode exhibits values of correlation coefficient as 0.9939, standard deviation as 0.0029 microA and limit of detection as 1.66 mM. UV-visible spectrophotometer studies reveal that 5.2x10(-3) U of ChOx are actively working per cm(2) area of ChOx/MEH-PPV/SA LB film bioelectrode and this bioelectrode is thermally stable upto 55 degrees C with reusability of about 60 times.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / methods*
  • Cholesterol / analysis*
  • Cholesterol Oxidase / metabolism*
  • Electrodes
  • Enzymes, Immobilized / metabolism*
  • Gold / chemistry
  • Membranes, Artificial*
  • Molecular Structure
  • Polymers / chemistry*
  • Sensitivity and Specificity
  • Stearic Acids / chemistry
  • Surface Properties
  • Vinyl Compounds / chemistry*

Substances

  • Enzymes, Immobilized
  • Membranes, Artificial
  • Polymers
  • Stearic Acids
  • Vinyl Compounds
  • poly(2-methoxy-5-(2'-ethylhexyloxy)-p-phenylenevinylene)
  • stearic acid
  • Gold
  • Cholesterol
  • Cholesterol Oxidase