Fast genomic muChIP-chip from 1,000 cells

John Arne Dahl; Andrew H Reiner; Philippe Collas

doi:10.1186/gb-2009-10-2-r13

Fast genomic muChIP-chip from 1,000 cells

Genome Biol. 2009 Feb 10;10(2):R13. doi: 10.1186/gb-2009-10-2-r13.

Authors

John Arne Dahl¹, Andrew H Reiner, Philippe Collas

Affiliation

¹ Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, 0317 Oslo, Norway.

Abstract

Genome-wide location analysis of histone modifications and transcription factor binding relies on chromatin immunoprecipitation (ChIP) assays. These assays are, however, time-consuming and require large numbers of cells, hindering their application to the analysis of many interesting cell types. We report here a fast microChIP (muChIP) assay for 1,000 cells in combination with microarrays to produce genome-scale surveys of histone modifications. muChIP-chip reliably reproduces data obtained by large-scale assays: H3K9ac and H3K9m3 enrichment profiles are conserved and nucleosome-free regions are revealed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Chromatin Immunoprecipitation / methods*
Genome, Human
Genomics / methods*
Histones / metabolism
Humans
Methods
Microarray Analysis / methods*
Protein Processing, Post-Translational

Substances

Histones