Abstract
Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Anthozoa / microbiology*
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Bacteria / genetics
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Bacteria / isolation & purification
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Bacterial Infections / microbiology
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Biodiversity
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification*
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DNA, Ribosomal / genetics
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DNA, Ribosomal / isolation & purification*
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Freezing*
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Molecular Sequence Data
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Phylogeny
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Polymerase Chain Reaction / methods*
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RNA, Ribosomal, 16S / genetics*
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Sequence Analysis, DNA
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Specimen Handling / methods*
Substances
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Ribosomal, 16S