Identification of a possible control element, Mt5, in the major noncoding region of mitochondrial DNA by intraspecific nucleotide conservation

K Ohno; M Tanaka; H Suzuki; T Ohbayashi; S Ikebe; H Ino; S Kumar; A Takahashi; T Ozawa

Identification of a possible control element, Mt5, in the major noncoding region of mitochondrial DNA by intraspecific nucleotide conservation

Biochem Int. 1991 May;24(2):263-72.

Authors

K Ohno¹, M Tanaka, H Suzuki, T Ohbayashi, S Ikebe, H Ino, S Kumar, A Takahashi, T Ozawa

Affiliation

¹ Department of Biomedical Chemistry, Faculty of Medicine, University of Nagoya, Japan.

PMID: 1930246

Abstract

Nucleotide sequences throughout the whole major noncoding region of mitochondrial DNA of 18 subjects were determined. Previously identified control elements were classified into three groups according to the degree of intraspecific nucleotide conservations: strictly conserved elements (LSP, HSP, Mt3, Mt3 on H-strand, mtTF1-element for HSP), relatively conserved elements (CSB-III, Mt4 on H-strand, and mtTF1-element for LSP), and variable elements (TAS, CSB-I, CSB-II). Moreover, alignment of nucleotide conservations disclosed a stretch of conserved sequence (5'-ATGCTTACAAGCAAG-3', nucleotide number 16, 194-16,208, designated as Mt5 element) in the middle of the hypervariable segment. Nucleotide conservation of this element was not only intraspecific but also interspecific.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Cell Cycle
DNA, Mitochondrial / chemistry
DNA, Mitochondrial / genetics*
DNA, Mitochondrial / isolation & purification
Gene Library
Humans
Molecular Sequence Data
Oligodeoxyribonucleotides
Polymerase Chain Reaction
Regulatory Sequences, Nucleic Acid*
Sequence Alignment

Substances

DNA, Mitochondrial
Oligodeoxyribonucleotides