Abstract
Surface charge switch of a 6-carboxyfluorescein (FAM) labeled lysozyme aptamer from negative to positive upon lysozyme binding triggers fluorescence resonance energy transfer (FRET) between an anionic conjugated polymer and the aptamer, which provides a convenient method for lysozyme detection in biological media with high sensitivity and selectivity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anions / chemistry
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Aptamers, Peptide / analysis*
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Aptamers, Peptide / chemistry
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Aptamers, Peptide / metabolism
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Fluorescence Resonance Energy Transfer / methods*
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Fluorescent Dyes / analysis*
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Fluorescent Dyes / chemistry*
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Molecular Structure
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Muramidase / analysis*
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Muramidase / chemistry*
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Muramidase / metabolism
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Polymers / chemistry*
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Sensitivity and Specificity
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Substrate Specificity
Substances
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Anions
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Aptamers, Peptide
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Fluorescent Dyes
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Polymers
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Muramidase