Fluorescence resonance energy transfer between an anionic conjugated polymer and a dye-labeled lysozyme aptamer for specific lysozyme detection

Chem Commun (Camb). 2009 May 7:(17):2284-6. doi: 10.1039/b820001g. Epub 2009 Mar 4.

Abstract

Surface charge switch of a 6-carboxyfluorescein (FAM) labeled lysozyme aptamer from negative to positive upon lysozyme binding triggers fluorescence resonance energy transfer (FRET) between an anionic conjugated polymer and the aptamer, which provides a convenient method for lysozyme detection in biological media with high sensitivity and selectivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anions / chemistry
  • Aptamers, Peptide / analysis*
  • Aptamers, Peptide / chemistry
  • Aptamers, Peptide / metabolism
  • Fluorescence Resonance Energy Transfer / methods*
  • Fluorescent Dyes / analysis*
  • Fluorescent Dyes / chemistry*
  • Molecular Structure
  • Muramidase / analysis*
  • Muramidase / chemistry*
  • Muramidase / metabolism
  • Polymers / chemistry*
  • Sensitivity and Specificity
  • Substrate Specificity

Substances

  • Anions
  • Aptamers, Peptide
  • Fluorescent Dyes
  • Polymers
  • Muramidase