Reverse genetics of Nipah virus to probe viral pathogenicity

Misako Yoneda; Kentaro Fujita; Hiroki Sato; Chieko Kai

doi:10.1007/978-1-59745-559-6_23

Reverse genetics of Nipah virus to probe viral pathogenicity

Methods Mol Biol. 2009:515:329-37. doi: 10.1007/978-1-59745-559-6_23.

Authors

Misako Yoneda¹, Kentaro Fujita, Hiroki Sato, Chieko Kai

Affiliation

¹ The University of Tokyo, Institute of Medical Science, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan.

PMID: 19378121
DOI: 10.1007/978-1-59745-559-6_23

Abstract

Enhanced green fluorescent protein (EGFP) is a useful marker protein which enables the tracing of virus infection. Recombinant viruses expressing EGFP are useful for the investigation of the underlying mechanism of viral infection in vitro and in vivo. Using EGFP-expressing recombinant Nipah virus (NiV) and canine distemper virus (CDV), we tested the susceptibility of a variety of cells to infection. Receptor usage in CDV infection was also investigated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal / immunology
Cell Line
Distemper Virus, Canine / genetics
Distemper Virus, Canine / pathogenicity
Genes, Reporter / genetics
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Humans
Nipah Virus / genetics*
Nipah Virus / pathogenicity*
Organ Specificity
Plasmids / genetics
Receptors, Virus / metabolism

Substances

Antibodies, Monoclonal
Receptors, Virus
enhanced green fluorescent protein
Green Fluorescent Proteins