Intracellular recording methods were used to examine stereotyped reflexes evoked in the circular muscle of guinea-pig small intestine by distension or repetitive deformation of the mucosal villi, in vitro. Both stimuli evoked compound excitatory junction potentials (EJPs) on the oral side of the site of stimulation and compound inhibitory junction potentials (IJPs) on the anal side. Stimulation of the mucosa by application of 10 microliters of 0.5 M HCl evoked similar reflex responses in the circular muscle. The compound EJPs evoked by mucosal stimulation were depressed, but not abolished, by 1 microM hyoscine, indicating that these responses were partially mediated by release of acetylcholine, as are the equivalent responses evoked by distension. The compound EJPs and the compound IJPs evoked by maintained distension or by repeated mechanical stimulation of the mucosa were transient, lasting in most cases for 3-5 s before the membrane potential returned to resting level. This decline (rundown) occurred in part of the circuit close to the site of stimulation as stimuli applied elsewhere during the period of rundown evoked normal EJPs and IJPs. Mechanical stimuli (brush strokes that deformed the mucosal villi) applied to the mucosa at the site of a maintained distension evoked responses of normal amplitude during the period when the response to the distension had declined to zero. In contrast, during the period when the responses to repetitive mechanical stimulation of the mucosa had disappeared, the reflex responses evoked by distension at the same site were substantially augmented. Chemical stimulation of the mucosa with acid also enhanced the responses to distension.(ABSTRACT TRUNCATED AT 250 WORDS)