Pros and cons of using resazurin staining for quantification of viable Staphylococcus aureus biofilms in a screening assay

J Microbiol Methods. 2009 Jul;78(1):104-6. doi: 10.1016/j.mimet.2009.04.014. Epub 2009 May 8.

Abstract

Staining of Staphylococcus aureus biofilms with 20 microM resazurin during 20 min was shown to provide a good screening assay in 96-well micro titer plates. However, data quality was found to be dependent on the staining duration and biofilm concentration. Also, the inadequacy of using resazurin calibration curves with planktonic cells to estimate S. aureus biofilm concentrations was demonstrated.

Publication types

  • Evaluation Study

MeSH terms

  • Biofilms*
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / metabolism
  • Microbial Viability*
  • Oxazines / chemistry*
  • Oxazines / metabolism
  • Staining and Labeling / methods*
  • Staphylococcus aureus / chemistry*
  • Staphylococcus aureus / physiology
  • Xanthenes / chemistry*
  • Xanthenes / metabolism

Substances

  • Fluorescent Dyes
  • Oxazines
  • Xanthenes
  • resazurin