Broad range RT-PCR assays targeting human noroviruses also detect swine noroviruses

Food Microbiol. 2009 Aug;26(5):552-5. doi: 10.1016/j.fm.2009.03.011. Epub 2009 Apr 5.

Abstract

Conventional broad range RT-PCR and real time RT-PCR assays designed to detect human noroviruses (NoVs) also efficiently detect swine NoVs. Investigation of the primers and probe binding sites revealed strong homologies between swine NoVs genomic sequences and human primer sequences. These findings have a serious impact on food diagnostic methods and laboratories.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Caliciviridae Infections / diagnosis*
  • Caliciviridae Infections / virology
  • DNA Primers
  • Food Contamination / analysis*
  • Food Microbiology
  • Gastroenteritis / diagnosis*
  • Gastroenteritis / virology
  • Gene Amplification
  • Humans
  • Norovirus / classification
  • Norovirus / isolation & purification*
  • RNA, Viral / analysis
  • Reverse Transcriptase Polymerase Chain Reaction* / methods
  • Reverse Transcriptase Polymerase Chain Reaction* / standards
  • Sensitivity and Specificity
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • Swine

Substances

  • DNA Primers
  • RNA, Viral