Highly sensitive mutation detection based on digital amplification coupled with hydrogel bead-array

Chem Commun (Camb). 2009 Jul 21:(27):4094-6. doi: 10.1039/b902196e. Epub 2009 Jun 10.

Abstract

In order to detect small amounts of mutants for early cancer diagnosis, we have developed the novel method of using amplicon-coated microbeads and single-molecule-PCR in water-in-oil emulsions, which we coupled with a new detection platform, the hydrogel bead-array, a 3-D polyacrylamide gel network used as a carrier to immobilize the beads.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Colorectal Neoplasms / diagnosis
  • Colorectal Neoplasms / genetics
  • DNA / genetics*
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Emulsions
  • Humans
  • Hydrogels / chemistry*
  • Microspheres
  • Mutation*
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity

Substances

  • Emulsions
  • Hydrogels
  • DNA