Objective: To investigate the telencephalon developmental characteristics of Hynobius leehii, and enrich the research data of comparable neurobiology and nervous system development of amphibian.
Methods: HE staining and Nissl staining methods were used to study the telencephalon histological structure of Hynobius leechii at both the metamorphosis and the adult phases, and to explore the developmental phases of telencephalon.
Results: The olfactory bulb could be roughly divided into 6 layers from lateral to medial. The lateral cerebral ventricles at the metamorphosis phase were smaller than those at the adult phase, and there were no clear borderlines between the primordial pallium and the primordial hippocampus, or between the primordial pallium and the primordial piriform area. Moreover, the cells in the primordial piriform area were more closely distributed than those in the primordial hippocampus or the primordial pallium. Compared with those at the adult phase, cells in nucleuses at the metamorphosis phase were larger in number and more closely distributed.
Conclusion: The telencephalon of Hynobius leehii at the metamorphosis phase has generally formed the adult structure. However, it is still at a transition state of differentiation to maturity during the development of Hynobius leehii.
目的: 探讨东北小鲵端脑发育的阶段性特点, 充实两栖类比较神经生物学和神经系统发育的研究资料。
方法: 采用HE染色法和尼氏染色法, 初步研究变态期和成体东北小鲵端脑的组织学结构特征, 探讨其端脑的部分发育过程。
结果: 嗅球结构由外侧向内侧大致分为6层。变态期东北小鲵端脑侧脑室腔小于成体东北小鲵, 并且其原始大脑皮层, 原始梨状区和原始海马的界限都不很清晰。原始梨状区的细胞分布与原始大脑皮层细胞和原始海马细胞相比, 更为密集。变态期各核团的细胞数量和细胞密集程度均显著大于成体水平。
结论: 变态期东北小鲵的端脑大致具备了成体结构, 但仍处于脑发育分化走向成熟的过渡阶段。