Nanostructured zinc oxide platform for mycotoxin detection

Bioelectrochemistry. 2010 Feb;77(2):75-81. doi: 10.1016/j.bioelechem.2009.06.014. Epub 2009 Jul 8.

Abstract

Nanostructured zinc oxide (Nano-ZnO) film has been deposited onto indium-tin-oxide (ITO) glass plate for co-immobilization of rabbit-immunoglubin antibodies (r-IgGs) and bovine serum albumin (BSA) for ochratoxin-A (OTA) detection. The results of X-ray diffraction (XRD) studies reveal the formation of Nano-ZnO with average particle size as ~5.0nm. Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) techniques have been used to characterize Nano-ZnO/ITO electrode and BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode. Electrochemical impedimetric response of BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode obtained as a function of OTA concentration exhibits linearity as 0.006-0.01nM/dm(3), detection limit of 0.006nM/dm(3), response time as 25s and sensitivity of 189Omega/nM/dm(3)cm(-2) with a regression coefficient of 0.997.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electric Impedance
  • Electrochemistry
  • Enzymes, Immobilized / chemistry*
  • Glass / chemistry*
  • Immunoglobulin G / immunology
  • Microelectrodes
  • Microscopy, Electron, Scanning
  • Mycotoxins / analysis*
  • Nanostructures / chemistry*
  • Ochratoxins / analysis
  • Particle Size
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Serum Albumin, Bovine / chemistry
  • Spectroscopy, Fourier Transform Infrared
  • Tin Compounds / chemistry*
  • X-Ray Diffraction
  • Zinc Oxide / chemistry*

Substances

  • Enzymes, Immobilized
  • Immunoglobulin G
  • Mycotoxins
  • Ochratoxins
  • Tin Compounds
  • Serum Albumin, Bovine
  • indium tin oxide
  • Zinc Oxide