Distribution of centromeric proteins and PARP-1 during mitosis and apoptosis

Ann N Y Acad Sci. 2009 Aug:1171:32-7. doi: 10.1111/j.1749-6632.2009.04729.x.

Abstract

A large complex of proteins, called CENPs, are associated with centromeric DNA. Some of them exhibit a cell cycle-related expression (e.g., CENP-E and -F) and are required for the transition from interphase to mitosis, whereas constitutive proteins (e.g., CENP-A, -B, -C, -G, and -H) reside permanently at the centromere and are essential for the correct kinetochore assembly. Poly(ADP-ribose) polymerase-1 (PARP-1), which plays an active role in many basic processes, was described as a possible regulator of CENPs. By multicolor immunofluorescence we therefore analyzed the distribution of PARP-1 and its interaction with CENP-B, -E, and -F during mitosis and apoptosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibiotics, Antineoplastic / pharmacology
  • Antineoplastic Agents, Phytogenic / pharmacology
  • Apoptosis / drug effects
  • Apoptosis / physiology*
  • Cell Nucleus / metabolism
  • Centromere Protein B / metabolism*
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Cytoplasm / metabolism
  • Dactinomycin / pharmacology
  • Etoposide / pharmacology
  • Fluorescent Antibody Technique
  • HeLa Cells
  • Humans
  • Immunohistochemistry
  • Microfilament Proteins / metabolism
  • Microscopy, Confocal
  • Microscopy, Fluorescence
  • Mitosis / physiology*
  • Poly (ADP-Ribose) Polymerase-1
  • Poly(ADP-ribose) Polymerases / metabolism*
  • Protein Binding

Substances

  • Antibiotics, Antineoplastic
  • Antineoplastic Agents, Phytogenic
  • Centromere Protein B
  • Chromosomal Proteins, Non-Histone
  • Microfilament Proteins
  • centromere protein E
  • centromere protein F
  • Dactinomycin
  • Etoposide
  • PARP1 protein, human
  • Poly (ADP-Ribose) Polymerase-1
  • Poly(ADP-ribose) Polymerases