The objective of this study was to compare the efficiency of pedaling (EP) and the electromyographic activity (EMG) between cyclists and non-cyclists during cycling in different cadences. Using a cyclosimulator, 12 cyclists (26.5 +/- 4.5 years; 68.2 +/- 10.5 kg; 175.6 +/- 8.2 cm) and 9 non-cyclists (25.1 +/- 4.3 years; 72.6 +/- 9.8 kg; 174.6 +/- 6.2 cm), performed a maximum incremental test (ITmax), and subsequently, two constant load tests (Tconst) in different cadences (60 and 90 rpm) at the intensity of the electromyographic fatigue threshold (EMGth) determined in ITmax. Before the Tconst, the subjects performed a maximum isometric voluntary contraction (MIVC) for the normalization of the EMG data of Tconst. During Tconst, the EMG of the studied muscles was recorded, as well as the EP Although there was a trend of higher values in all occasions for the cyclists, there were no statistical differences in EP and the EMG when compared in a same cadence between groups. However, when the EMG is compared in different cadences in the same group, there was a significant increase (p < 0.05) in the muscles that work during the recovery phase with the increase in cadence, in both groups, being more evident in the cyclists. In conclusion, the hypothesis that cyclists had better technique than non-cyclists was not confirmed statistically. However, it was found that the increase in cadence improves the EP and the recruitment in both groups.