Enhanced amplification of GC-rich DNA with two organic reagents

Biotechniques. 2009 Sep;47(3):775-9. doi: 10.2144/000113203.

Abstract

Amplification of GC-rich DNA sequences is still a difficult task worldwide. Two frequently seen and inexpensive reagents-ethylene glycol and 1,2-propanediol-were found to be more effective than betaine in the amplification of 104 randomly selected GC-rich human DNA sequences with GC contents of 60-80% and lengths of 700-800 bp.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ethylene Glycol*
  • GC Rich Sequence*
  • Humans
  • Nucleic Acid Amplification Techniques / methods*
  • Propylene Glycol*
  • Sequence Analysis, DNA / methods*

Substances

  • Propylene Glycol
  • Ethylene Glycol