Abstract
A temperature-sensitive mutant of p53, p53Val-135, was found to be able to arrest cell proliferation when overexpressed at 32.5 degrees C. While much of the protein was cytoplasmic in cells proliferating at 37.5 degrees C, it became predominantly nuclear at 32.5 degrees C. Concomitantly, p53Val-135 became destabilized, although not to the extent seen in primary fibroblasts.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Compartmentation
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Cell Division*
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Cell Nucleus / metabolism
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Cell Transformation, Neoplastic / pathology
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Fluorescent Antibody Technique
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In Vitro Techniques
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Mice
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Mutation
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Temperature
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Tumor Suppressor Protein p53 / physiology*
Substances
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Tumor Suppressor Protein p53