PBS incubation, a simple technique to release miracidia of Opisthorchis-like eggs for DNA extraction

Saiwasan Buathong; Mathirut Mungthin; Peerapan Tan-ariya; Tawee Naaglor; Paanjit Taamasri; Picha Suwannahitatorn; Saovanee Leelayoova

doi:10.1016/j.exppara.2009.11.011

PBS incubation, a simple technique to release miracidia of Opisthorchis-like eggs for DNA extraction

Exp Parasitol. 2010 Mar;124(3):357-9. doi: 10.1016/j.exppara.2009.11.011. Epub 2009 Dec 2.

Authors

Saiwasan Buathong¹, Mathirut Mungthin, Peerapan Tan-ariya, Tawee Naaglor, Paanjit Taamasri, Picha Suwannahitatorn, Saovanee Leelayoova

Affiliation

¹ Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

PMID: 19961851
DOI: 10.1016/j.exppara.2009.11.011

Abstract

This study describes a simple technique for releasing miracidia from Opisthorchis-like eggs before DNA extraction by keeping PBS washed specimens at 4 degrees C for two days. Using PCR amplification, the sensitivity of the PBS incubation technique was as good as that obtained from freeze-thaw technique. Moreover, this low-cost technique is less laborious.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cold Temperature
DNA, Helminth / isolation & purification*
Feces / parasitology*
Humans
Opisthorchis / genetics
Opisthorchis / isolation & purification
Opisthorchis / physiology*
Ovum / physiology
Polymerase Chain Reaction
Sensitivity and Specificity
Sodium Chloride
Time Factors

Substances

DNA, Helminth
Sodium Chloride