Herpes simplex virus type 2 (HSV-2) causes a chronic infection that is recognised as the leading cause of genital ulcer disease worldwide and is known to increase the risk of HIV infection. In a cross-sectional study we examined risk factors for HSV-2 among 176 pregnant teenagers recruited from three primary health care clinics in Zimbabwe. The prevalence of HSV-2 and HIV were 41.6% and 29.2% respectively. HIV-infected teenagers were more likely to be HSV-2 seropositive compared with the HIV uninfected teenagers, odds ratio (OR) 7.9 (95% confidence interval (CI) 3.7-16.9). In multivariate analysis having an older partner remained independently associated with HSV-2 seropositivity, OR 2.9 (95% CI 1.2-6.9) suggesting that risk factors for HSV-2 seropositivity among pregnant teenagers depend primarily on the behaviour of the male partners.