Crystal structure of PsbQ from Synechocystis sp. PCC 6803 at 1.8 A: implications for binding and function in cyanobacterial photosystem II

Biochemistry. 2010 Apr 6;49(13):2765-7. doi: 10.1021/bi100217h.

Abstract

In Synechocystis sp. PCC 6803, PsbQ is associated with photosystem II (PSII) complexes with the highest activity and stability. However, this subunit is not found in PSII X-ray crystallographic structures from Thermosynechococcus elongatus or Thermosynechococcus vulcanus. We present the crystal structure of cyanobacterial PsbQ determined in the presence and absence of Zn(2+). The protein has a well-defined helical core, containing four helices arranged in an up-down-up-down fold. A conserved potential interaction site composed of a divalent metal binding site and adjacent hydrophobic pocket has been identified.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Binding Sites
  • Crystallography, X-Ray
  • Cyanobacteria
  • Photosystem II Protein Complex / chemistry*
  • Protein Binding
  • Protein Conformation
  • Protein Subunits / chemistry
  • Synechocystis / chemistry*
  • Zinc

Substances

  • Bacterial Proteins
  • Photosystem II Protein Complex
  • Protein Subunits
  • Zinc

Associated data

  • PDB/3LS0
  • PDB/3LS1