Histologic and ultrastructural features of cryopreserved ovine ovarian tissue: deleterious effect of 1,2-propanediol applying different thawing protocols

Irma C Oskam; Babak A Asadi; Regiane R Santos

doi:10.1016/j.fertnstert.2010.02.003

Histologic and ultrastructural features of cryopreserved ovine ovarian tissue: deleterious effect of 1,2-propanediol applying different thawing protocols

Fertil Steril. 2010 May 15;93(8):2764-6. doi: 10.1016/j.fertnstert.2010.02.003. Epub 2010 Mar 16.

Authors

Irma C Oskam¹, Babak A Asadi, Regiane R Santos

Affiliation

¹ Department of Obstetrics and Gynecology, Oslo University Hospital, Oslo, Norway. icaoska@smartcall.no

PMID: 20236634
DOI: 10.1016/j.fertnstert.2010.02.003

Abstract

Ovarian cortical fragments were frozen in presence of 1.5 M PROH, thawed at different temperatures (37 degrees, 30 degrees, or 4 degrees C) and submitted to histologic and ultrastructural analysis. Thawing at 30 degrees C minimized stromal and follicular damage when compared with 4 degrees and 37 degrees C.

MeSH terms

Animals
Cryopreservation / methods*
Cryoprotective Agents / pharmacology*
Female
Ovary / drug effects*
Ovary / ultrastructure
Propylene Glycol / pharmacology
Sheep
Temperature

Substances

Cryoprotective Agents
Propylene Glycol