Molecular inference of phylogenetic relationships among Decapodiformes (Mollusca: Cephalopoda) with special focus on the squid order Oegopsida

Abstract

Squids, cuttlefish and bobtail squids comprise the molluscan superorder Decapodiformes (Mollusca: Cephalopoda). Although these animals exemplify the morphological and ecological diversity seen in Cephalopoda, no previous study has focused resolving decapodiform relationships, particularly within Oegopsida, a large order comprised of pelagic squid. To further clarify the phylogenetic history of Decapodiformes, and Oegopsida in particular, molecular data for five genes (18S rRNA, 28S rRNA, Histone H3, 16S rRNA, COI) was collected for 90 taxa representing all major lineages and families and evaluated using parsimony, likelihood, and Bayesian analysis. Although ordinal relationships were sensitive to analytical method, several conclusions can be inferred: the pelagic order Myopsida is closely related to the benthic sepioids, whose relationships were ambiguous, and Bathyteuthoidea is distinct from Oegopsida. Within Oegopsida several clades are consistently recovered, some with previous morphological support (e.g. chiroteuthid, lepidoteuthid, histioteuthid families) while others suggest novel relationships (e.g. Architeuthidae+Neoteuthidae). This study, with its broad coverage of taxa, provides the first in-depth analysis of Decapodiformes with special focus on the morphologically and biogeographically diverse Oegopsida, confirms several sister-taxon relationships, and provides new hypotheses of cephalopod evolution in the open ocean.