MASPECTRAS 2 is a freely available platform for integrating MS protein identifications with information from the major bioinformatics databases (ontologies, domains, literature, etc.). It assists researchers in understanding their data and publishing through sample comparisons, targeted queries, summaries, and exports in multiple formats such as PRIDE XML (Jones et al., Nucleic Acids Res. 2008, 36, D878-D883). MASPECTRAS 2 also comprises mechanisms to facilitate its integration in a high-throughput infrastructure. We illustrate application of MASPECTRAS 2 with unpublished tyrosine kinase inhibitor drug proteomics profiles in cancerous cells.