Antioxidants, Oxyrase, and mitochondrial uncoupler 2,4-dinitrophenol improved postthaw survival of rhesus monkey sperm from ejaculates with low cryosurvival

Fertil Steril. 2010 Nov;94(6):2359-61. doi: 10.1016/j.fertnstert.2010.04.017.

Abstract

Various antioxidant strategies such as supplementation of antioxidants, limiting oxygen concentration with Oxyrase, and reducing reactive oxygen species through mild mitochondrial uncoupling had statistically significant beneficial effects on sperm cryopreservation from rhesus monkeys with low cryoresistant ejaculates. Individuals or species that have higher sensitivity to cryodamage may derive the most benefit from these treatments.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • 2,4-Dinitrophenol / pharmacology*
  • Animals
  • Antioxidants / pharmacology*
  • Cell Survival / drug effects
  • Cryopreservation / methods
  • Ejaculation / physiology
  • Freezing
  • Macaca mulatta* / metabolism
  • Macaca mulatta* / physiology
  • Male
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • Oxygenases / pharmacology*
  • Reactive Oxygen Species / metabolism
  • Semen Analysis
  • Semen Preservation / methods
  • Spermatozoa / drug effects*
  • Spermatozoa / metabolism
  • Spermatozoa / physiology
  • Uncoupling Agents / pharmacology

Substances

  • Antioxidants
  • Reactive Oxygen Species
  • Uncoupling Agents
  • Oxygenases
  • Oxyrase
  • 2,4-Dinitrophenol