Abstract
In this study, the concept of size-selective proteolysis was first described by using the mesoporous silica-based trypsin nanoreactor. For analysis of a complex protein sample, low-MW proteins were preferentially digested for identification while high-MW proteins were excluded from digestion.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Enzymes, Immobilized / chemistry*
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Microscopy, Electron, Transmission
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Molecular Weight
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Nanostructures / chemistry*
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Particle Size
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Porosity
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Proteome / analysis*
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Proteome / chemistry
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Proteomics / instrumentation
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Proteomics / methods*
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Serum Albumin, Bovine / chemistry
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Silicon Dioxide / chemistry*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Surface Properties
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Trypsin / chemistry*
Substances
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Enzymes, Immobilized
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Proteome
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Serum Albumin, Bovine
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Silicon Dioxide
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Trypsin