Size-selective proteolysis on mesoporous silica-based trypsin nanoreactor for low-MW proteome analysis

Chem Commun (Camb). 2010 Sep 7;46(33):6144-6. doi: 10.1039/c0cc00619j. Epub 2010 Jul 27.

Abstract

In this study, the concept of size-selective proteolysis was first described by using the mesoporous silica-based trypsin nanoreactor. For analysis of a complex protein sample, low-MW proteins were preferentially digested for identification while high-MW proteins were excluded from digestion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Enzymes, Immobilized / chemistry*
  • Microscopy, Electron, Transmission
  • Molecular Weight
  • Nanostructures / chemistry*
  • Particle Size
  • Porosity
  • Proteome / analysis*
  • Proteome / chemistry
  • Proteomics / instrumentation
  • Proteomics / methods*
  • Serum Albumin, Bovine / chemistry
  • Silicon Dioxide / chemistry*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Surface Properties
  • Trypsin / chemistry*

Substances

  • Enzymes, Immobilized
  • Proteome
  • Serum Albumin, Bovine
  • Silicon Dioxide
  • Trypsin