The traditional nonabsorbable silicone buckling implant (buckle) may cause some long-term complications when it is used in the retinal detachment surgery. In this study we fabricated a chitosan-gelatin based buckling implant to replace the traditional one. We evaluated its biocompatibility with human scleral fibroblasts (HSF) in vitro and its cytotoxicity with L929 cell. To evaluate elasticity and hardness of the blends buckle, the mechanical properties of the chitosan-gelatin buckle were compared with the traditional silicone buckle. The light and electron microscopy coupled with immunocytochemistry demonstrated that chitosan-gelatin blends supported the survival and growth of primarily cultured HSF without significant cytotoxic effects. MTT analysis and cell cycle analysis indicated that chitosan-gelatin blends promoted the proliferation of HSF. A preliminary in vivo implantation test indicated that chitosan-gelatin buckling implant were compatible with the surrounding tissue. The results collectively demonstrated that chitosan-gelatin blends could be a candidate biodegradable material for scleral buckling surgery.