Cell wall anchoring of the 37-kilodalton oncofetal antigen by Lactobacillus plantarum for mucosal cancer vaccine delivery

Lasse Fredriksen; Geir Mathiesen; Mouldy Sioud; Vincent G H Eijsink

doi:10.1128/AEM.01031-10

Cell wall anchoring of the 37-kilodalton oncofetal antigen by Lactobacillus plantarum for mucosal cancer vaccine delivery

Appl Environ Microbiol. 2010 Nov;76(21):7359-62. doi: 10.1128/AEM.01031-10. Epub 2010 Sep 17.

Authors

Lasse Fredriksen¹, Geir Mathiesen, Mouldy Sioud, Vincent G H Eijsink

Affiliation

¹ Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, N-1432 Ås, Norway.

Abstract

The 37-kDa oncofetal antigen (OFA), a tumor immunogen expressed on all mammalian cancers examined to date, was secreted and anchored to the cell wall of Lactobacillus plantarum using homologous signal peptides and LPxTG anchors. Orally administered L. plantarum expressing anchored OFA induced a specific immune response against OFA in mice.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antibodies, Neoplasm / immunology
Antigens, Neoplasm / genetics
Antigens, Neoplasm / immunology
Antigens, Neoplasm / metabolism*
Blotting, Western
Cancer Vaccines / administration & dosage
Cancer Vaccines / immunology
Cancer Vaccines / metabolism*
Cell Wall / immunology
Cell Wall / metabolism*
Female
Lactobacillus plantarum / immunology
Lactobacillus plantarum / metabolism*
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Neoplasms, Experimental / immunology
Recombinant Proteins / genetics

Substances

Antibodies, Neoplasm
Antigens, Neoplasm
Cancer Vaccines
Recombinant Proteins
oncofetal antigens