Exercise improves bladder function in diabetic mice

Abstract

Aims: We determined the effect of exercise on bladder dysfunction and voiding frequency in db/db mice.

Materials and methods: Diabetic db/db female mice (BKS.Cg-Dock7m +/+ Leprdb/J strain) and their age-matched wild-type controls (WT) were equally divided into sedentary and exercise groups. Mice were exercised for 1 hr everyday for 8 weeks (speed of 5.2 m/min). We performed a voiding pattern test, cystometric analysis and reactivity of isolated bladder strips in WT and db/db mice, both sedentary and exercised.

Results: Diabetes increased the frequency of voiding, bladder capacity, and residual volume. Exercise decreased voiding frequency in db/db mice; voiding frequency was 5.8 ± 0.5 (db/db exercise) versus 10.8 ± 1.1 (db/db control, P < 0.001). In cystometric analysis, the bladder capacity of db/db sedentary mice was 0.27 ± 0.05 ml and was 0.14 ± 0.02 ml in the db/db exercise group (P < 0.05), whereas the residual volume was 0.2 ± 0.03 ml in db/db sedentary mice and 0.06 ± 0.02 ml in db/db Ex mice. Isolated strips of bladder muscle from sedentary db/db mice were more responsive to carbachol than strips from db/db exercise mice. Exercise did not improve the urodynamic properties of WT mice, both sedentary and exercised.

Conclusions: Exercise improves bladder function in diabetic mice by reducing voiding frequency and improving urodynamic parameters.