Five-minute Giemsa stain for rapid detection of malaria parasites in blood smears

M M Jager; J L Murk; R D Piqué; T A M Hekker; C M J E Vandenbroucke-Grauls

doi:10.1258/td.2010.100218

Five-minute Giemsa stain for rapid detection of malaria parasites in blood smears

Trop Doct. 2011 Jan;41(1):33-5. doi: 10.1258/td.2010.100218. Epub 2010 Nov 18.

Authors

M M Jager¹, J L Murk, R D Piqué, T A M Hekker, C M J E Vandenbroucke-Grauls

Affiliation

¹ Department of Medical Microbiology and Infection Control, VU University Medical Center, De Boelelaan 1117, Amsterdam 1081 HV, The Netherlands. m.jager@vumc.nl

PMID: 21088023
DOI: 10.1258/td.2010.100218

Abstract

The Giemsa stain is used as the gold standard for the diagnosis of malaria on blood smears. The classical staining procedure requires between 30 and 45 min. We modified the Giemsa stain and reduced the staining time to 5 min without any loss of quality.

Publication types

Evaluation Study

MeSH terms

Animals
Azure Stains*
Blood Specimen Collection / methods
Coloring Agents*
Humans
Malaria / blood
Malaria / diagnosis*
Malaria / parasitology
Parasitemia / blood
Parasitemia / diagnosis
Parasitemia / parasitology
Plasmodium falciparum / isolation & purification*
Plasmodium ovale / isolation & purification*
Plasmodium vivax / isolation & purification*
Sensitivity and Specificity
Staining and Labeling / methods
Time Factors

Substances

Azure Stains
Coloring Agents