Abstract
A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Alleles
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Animals
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Bacterial Proteins / genetics
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Bacterial Typing Techniques
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Burkholderia mallei / classification
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Burkholderia mallei / genetics*
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Burkholderia mallei / isolation & purification
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Burkholderia pseudomallei / classification
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Burkholderia pseudomallei / genetics*
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Burkholderia pseudomallei / isolation & purification
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DNA, Bacterial / genetics*
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Diagnosis, Differential
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Genome, Bacterial / genetics
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Glanders / diagnosis
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Glanders / microbiology
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Humans
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Melioidosis / diagnosis
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Melioidosis / microbiology
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Polymerase Chain Reaction / methods*
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Polymorphism, Single Nucleotide
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Reproducibility of Results
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Sensitivity and Specificity
Substances
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Bacterial Proteins
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DNA, Bacterial