Comparison of PCR with culture applied on nasopharyngeal and throat swab specimens for the detection of Bordetella pertussis

Mona Holberg-Petersen; Pål A Jenum; Turid Mannsåker; Kjetil K Melby

doi:10.3109/00365548.2010.538855

Comparison of PCR with culture applied on nasopharyngeal and throat swab specimens for the detection of Bordetella pertussis

Scand J Infect Dis. 2011 Mar;43(3):221-4. doi: 10.3109/00365548.2010.538855. Epub 2010 Nov 25.

Authors

Mona Holberg-Petersen¹, Pål A Jenum, Turid Mannsåker, Kjetil K Melby

Affiliation

¹ Department of Microbiology, Oslo University Hospital, Ullevål, Norway. mona.holberg-petersen@uus.no

PMID: 21108541
DOI: 10.3109/00365548.2010.538855

Abstract

An in-house nested polymerase chain reaction (PCR) was prospectively compared with culture for Bordetella pertussis detection in 435 nasopharyngeal and/or throat swabs from 304 patients. One hundred specimens - 21% of nasopharyngeal swabs and 25% of throat swabs - were PCR- and/or culture-positive. Seventy percent of positive nasopharyngeal samples and 44% of positive throat samples were culture-positive.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Adolescent
Adult
Aged
Bacteriological Techniques / methods*
Bordetella pertussis / genetics
Bordetella pertussis / growth & development
Bordetella pertussis / isolation & purification*
Child
Child, Preschool
Humans
Infant
Infant, Newborn
Middle Aged
Nasopharynx / microbiology*
Pharynx / microbiology*
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Young Adult