Photoactivable peptides for identifying enzyme-substrate and protein-protein interactions

Chem Commun (Camb). 2011 Feb 7;47(5):1488-90. doi: 10.1039/c0cc04457a. Epub 2010 Dec 13.

Abstract

Photoactivated cross-linking of peptides to proteins is a useful strategy for identifying enzyme-substrate and protein-protein interactions in cell lysates as demonstrated by studies on the human hypoxia inducible factor system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Cross-Linking Reagents / pharmacology*
  • Humans
  • Hypoxia-Inducible Factor 1, alpha Subunit / chemistry
  • Hypoxia-Inducible Factor 1, alpha Subunit / metabolism*
  • Kidney / cytology
  • Kidney / metabolism
  • Molecular Probes
  • Peptide Fragments / chemistry
  • Peptide Fragments / radiation effects*
  • Photochemistry*
  • Protein Interaction Mapping
  • Spectrometry, Mass, Electrospray Ionization
  • Von Hippel-Lindau Tumor Suppressor Protein / chemistry
  • Von Hippel-Lindau Tumor Suppressor Protein / metabolism*

Substances

  • Cross-Linking Reagents
  • HIF1A protein, human
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Molecular Probes
  • Peptide Fragments
  • Von Hippel-Lindau Tumor Suppressor Protein
  • VHL protein, human