This study determined emm subtypes of Streptococcus pyogenes strains isolated from asymptomatic carriers and children with pharyngitis. All strains were previously investigated for fibronectin-binding genes (prtF1 and prtF2) and antimicrobial susceptibility. The most significant differences between the two groups, which share only 5 of the 14 detected emmsubtypes, concern the presence of the two more common emmsubtypes, 12.0 (50.0% vs. 3.1%, for asymptomatic carriers and children with pharyngitis, respectively) and 1.0 (28.1% vs. 0%, for children with pharyngitis and asymptomatic carriers, respectively).