The properties and applications of single-molecule DNA sequencing

Genome Biol. 2011;12(2):217. doi: 10.1186/gb-2011-12-2-217. Epub 2011 Feb 24.

Abstract

Single-molecule sequencing enables DNA or RNA to be sequenced directly from biological samples, making it well-suited for diagnostic and clinical applications. Here we review the properties and applications of this rapidly evolving and promising technology.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • DNA / analysis*
  • DNA / genetics
  • DNA Primers / chemistry
  • DNA Primers / genetics
  • DNA-Directed DNA Polymerase / genetics
  • DNA-Directed DNA Polymerase / metabolism
  • Fluorescence
  • Fluorescence Resonance Energy Transfer
  • Genomics / instrumentation
  • Genomics / methods*
  • High-Throughput Nucleotide Sequencing / instrumentation
  • High-Throughput Nucleotide Sequencing / methods*
  • Humans
  • Molecular Probes / chemistry
  • Molecular Probes / metabolism
  • Nanotechnology / instrumentation
  • Nanotechnology / methods*
  • Quantum Dots
  • RNA / analysis*
  • RNA / genetics
  • Sequence Analysis, DNA / instrumentation
  • Sequence Analysis, DNA / methods*

Substances

  • DNA Primers
  • Molecular Probes
  • RNA
  • DNA
  • DNA-Directed DNA Polymerase