Abstract
4-Coumarate:CoA ligase 2 (4CL2) from Arabidopsis thaliana catalyzes the ATP-dependent formation of the 4-coumaroyl-CoA thioester through the formation of 4-coumarate-AMP. Recombinant 4CL2 protein was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P2(1), with unit-cell parameters a=91.6, b=55.5, c=124.4 Å, α=γ=90.0, β=111.1°.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acyl Coenzyme A / chemistry
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Acyl Coenzyme A / metabolism
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Arabidopsis / enzymology*
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Arabidopsis Proteins / chemistry*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism
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Coenzyme A Ligases / chemistry*
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Coenzyme A Ligases / genetics
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Coenzyme A Ligases / metabolism
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Crystallization
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Crystallography, X-Ray
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Molecular Sequence Data
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
Substances
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Acyl Coenzyme A
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Arabidopsis Proteins
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Recombinant Proteins
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4-coumaroyl-coenzyme A
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Coenzyme A Ligases
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4-coumarate-CoA ligase