Abstract
Loss of adipose tissue in patients with pancreatic cancer may involve altered gene expression. Peri-operative mRNA levels of 44 genes were analysed by RT-PCR in intra-abdominal (IAAT) and subcutaneous adipose tissue (SCAT) sampled from pancreatic ductal adenocarcinoma (PDAC) patients undergoing tumour resection (n = 20), and control patients without cancer (n = 11). Peri- and post-operative IAAT and SCAT masses were measured by computerized tomography. PDAC patients displayed 2.6- and 1.7-fold higher Zn-α2-glycoprotein (AZGP1) mRNA levels than controls in IAAT and SCAT, respectively (P < 0.01), but expression was not correlated with post-operative changes in fat masses. IAAT mass changes correlated with genes in lipid metabolism, inflammation and apoptosis: e.g. stearoyl-Coenzyme A desaturase 1 (SCD), tumour necrosis factor (TNF) and chemokine (C-C motif) ligand 2 (CCL2; MCP-1). Patients with PDAC displayed increased AZGP1 mRNA levels in both IAAT and SCAT, but expression of other genes may predict IAAT loss.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipokines
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Aged
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Animals
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Apoptosis / genetics
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Cachexia / metabolism
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Carcinoma, Pancreatic Ductal / genetics
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Carcinoma, Pancreatic Ductal / metabolism*
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Carcinoma, Pancreatic Ductal / pathology
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Case-Control Studies
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Chemokine CCL2 / genetics
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Chemokine CCL2 / metabolism
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Fats / chemistry
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Fats / metabolism
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Female
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Gene Expression Regulation, Neoplastic*
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Glycoproteins / genetics
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Glycoproteins / metabolism
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Humans
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Intra-Abdominal Fat / chemistry
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Intra-Abdominal Fat / metabolism
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Lipid Metabolism / genetics*
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Male
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Mice
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Middle Aged
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Pancreatic Neoplasms / genetics
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Pancreatic Neoplasms / metabolism*
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Pancreatic Neoplasms / pathology
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Polymerase Chain Reaction
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RNA, Messenger
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Stearoyl-CoA Desaturase / genetics
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Stearoyl-CoA Desaturase / metabolism
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Subcutaneous Fat, Abdominal / chemistry
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Subcutaneous Fat, Abdominal / metabolism
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Tomography, Emission-Computed
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Tumor Necrosis Factor-alpha / genetics
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Tumor Necrosis Factor-alpha / metabolism
Substances
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AZGP1 protein, human
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Adipokines
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Carrier Proteins
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Chemokine CCL2
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Fats
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Glycoproteins
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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SCD1 protein, human
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Stearoyl-CoA Desaturase