Radon risk assessment is carried out by means of accurate measurements with active or passive instrumentation. All radon detectors must be calibrated and tested using a radon chamber containing a known concentration of radon produced by specific sources of (226)Ra. Some chambers can also be used to test the response of detectors as a function of environmental conditions. In this case, a calibration curve can be inferred with respect to change in one of the considered parameters. For this aim, a new radon chamber was designed and realised to perform calibration and to study the detector response in a large range of variation of the environmental parameters (pressure, 700-1100 mbar; temperature, 5-50°C; humidity, 10-90 %). The first experiments conducted to study the influence of environmental parameters on the detector response have shown flexibility and ease of use of the chamber.