[High-resolution melting: a analysis for genotyping of MDR1 C3435T in benzene-exposed workers]

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2011 Jan;29(1):70-2.
[Article in Chinese]

Abstract

Objective: Using high resolution melting (HRM) to analysis MDR1 C3435T in people exposed to benzene.

Methods: Restriction fragment length polymorphism (RFLP) was utilized to detect the polymorphism of MDR1 3435 in 121 benzene-exposed workers, and the results were compared with the HRM in 10% samples and were confirmed with direct sequencing for six people in them.

Results: By direct sequencing, consistent results of benzene-exposed workers with RFLP or HRM were got. The new high resolution melting curve analysis is more efficient, more convenient, and cheaper than RFLP.

Conclusion: High-resolution melting analysis provides a valid approach to efficiently detect DNA genetic diagnosis, which is suitable for detect susceptible genes in occupational surveillance.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics*
  • Benzene*
  • Genotype
  • Genotyping Techniques / methods*
  • Heterozygote
  • Humans
  • Occupational Exposure*
  • Polymorphism, Restriction Fragment Length
  • Polymorphism, Single Nucleotide*

Substances

  • ABCB1 protein, human
  • ATP Binding Cassette Transporter, Subfamily B
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Benzene