Full sequence analysis of the original Sapporo virus

Microbiol Immunol. 2011 Sep;55(9):657-60. doi: 10.1111/j.1348-0421.2011.00358.x.

Abstract

In this study, the full-length genome sequence of the prototype of sapovirus, namely Sapporo virus (SV82), was identified. Sapporo virus RNA was extracted from a fecal sample, amplified by RT-PCR and the PCR products sequenced directly and analyzed. Sequence analysis showed that Sapporo virus consists of 7433 nucleotides and has three open reading frames. The Sapporo strain shows 91.7% nucleotide sequence identity to the Manchester virus. Phylogenic analysis has also revealed the closeness of Sapporo virus to other sapovirus/genogroup I strains. Basic information on the evolutionary history of sapovirus analysis is provided here.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cluster Analysis
  • Feces / virology
  • Genome, Viral*
  • Humans
  • Molecular Sequence Data
  • Open Reading Frames
  • Phylogeny
  • RNA, Viral / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sapovirus / genetics*
  • Sapovirus / isolation & purification
  • Sequence Analysis, DNA*
  • Sequence Homology, Nucleic Acid

Substances

  • RNA, Viral

Associated data

  • GENBANK/HM002617