Aim: To explore the effect of lincomycin (lin) on the immune function of dendritic cell (DCs) line DC2.4.
Methods: Three experimental groups, namely, DC2.4 cells group, DC2.4 Cells+LPS group and DC2.4 cells +LPS +lin group were established (LPS and lin were 500 ng/mL). The morphological changes in each group were observed under inverted microscope. The MHC class II , CD86 and CD80 on the DC2.4 cells were detected by flow cytometer. The effects of lipopolysaccharide (LPS) and LPS +lin on the DC2.4 cells immuno-stimulatory capacity were evaluated by allogeneic mixed leukocytes reaction (MLR) between DC2.4 cells and T cells. ELISA was adopted to quantitate the level of IFN-γin the supernatant of cultured DC2.4 cells and T cells in each group.
Results: DC2.4 cells showed typical morphology of immature DCs. When stimulated with LPS or LPS combined with lin, DC2.4 cells exhibited typical maturate DCs modality. LPS of 500 ng/mL could significantly up-regulate the expression of MHC class II , CD86 and CD80 on DC2.4 cells and also augment stimulatory action of DC2.4 cells on T cells proliferation and secretion of IFN-γ. However, compared with LPS alone, the treatment of lin (500 ng/mL) combined with LPS down-regulated the immmuno-regulation function of DC2.4 cells.
Conclusion: Lin can partly inhibit the immuno-regulation function of maturate DC2.4 cells.