Abstract
Escherichia coli nucleoside diphosphate (NDP) kinase mutants have an increased frequency of spontaneous mutation, possibly due to uracil misincorporation into DNA. Here we show that NDP kinase mutants are dependent on translesion DNA synthesis, often a mutagenic form of DNA synthesis, to prevent mutagenesis.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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DNA Damage
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DNA Replication*
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DNA, Bacterial / genetics*
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DNA, Bacterial / metabolism
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DNA-Directed DNA Polymerase / metabolism
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Gene Expression Regulation, Bacterial
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Mutagenesis*
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Mutation
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Nucleoside-Diphosphate Kinase / genetics
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Nucleoside-Diphosphate Kinase / metabolism*
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Plasmids
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Uracil / metabolism
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beta-Galactosidase / metabolism
Substances
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DNA, Bacterial
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Escherichia coli Proteins
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Uracil
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Nucleoside-Diphosphate Kinase
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DNA-Directed DNA Polymerase
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beta-Galactosidase