Individual recognition has been studied across a number of taxa and modalities; however, few attempts have been made to combine chemical and biological approaches and arrive at a more complete understanding of the use of secretions as signals. We combined behavioral habituation experiments with gas chromatography-mass spectrometry of glandular secretions from the left and right flank gland and midventral gland of the rat-like hamster, Tscheskia triton. We found that females became habituated to one scent and then could discriminate individuals via another scent source from the same individual only when familiar with the scent donor. However, this prior social interaction was not required for females to discriminate different individuals in single-stimulus habituation-dishabituation tests. Chemical analyses revealed a similarity in volatile compounds between the left and right flank gland and midventral gland scents. It appears that individually distinctive cues are integratively coded by a combination of both flank gland and midventral gland secretions, instead of a single scent, albeit animals show different preferences to the novel scent. Our results suggest that odors from the flank and midventral glands may provide information related to individuality and aid individual recognition in this species and confirm that prior interaction between individuals is a prerequisite for rat-like hamsters to form multi-odor memory of a particular conspecific.