Saccharomyces cerevisiae homoserine kinase is homologous to prokaryotic homoserine kinases

N P Schultes; A D Ellington; J M Cherry; J W Szostak

doi:10.1016/0378-1119(90)90250-u

Saccharomyces cerevisiae homoserine kinase is homologous to prokaryotic homoserine kinases

Gene. 1990 Dec 15;96(2):177-80. doi: 10.1016/0378-1119(90)90250-u.

Authors

N P Schultes¹, A D Ellington, J M Cherry, J W Szostak

Affiliation

¹ Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.

PMID: 2176637
DOI: 10.1016/0378-1119(90)90250-u

Abstract

The Saccharomyces cerevisiae gene (THR1) encoding homoserine kinase (HK; EC 2.7.1.39) was cloned by complementation in yeast. Disruption of the THR1 gene results in threonine auxotrophy in yeast. Comparison of the amino acid sequences of yeast and bacterial HKs reveals substantial similarity.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacteria / enzymology
Bacteria / genetics
Base Sequence
Genes, Fungal
Genetic Complementation Test
Molecular Sequence Data
Phosphotransferases (Alcohol Group Acceptor)*
Phosphotransferases / genetics*
Phosphotransferases / metabolism
Saccharomyces cerevisiae / enzymology
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / physiology
Sequence Homology, Nucleic Acid
Threonine / metabolism

Substances

Threonine
Phosphotransferases
Phosphotransferases (Alcohol Group Acceptor)
homoserine kinase

Associated data

GENBANK/M37692