Abstract
Nonenzymatic glycation of proteins is associated with the long-term diabetes complication. The aim of this work was to examine in vitro the infuence of metformin on glycated proteins formation by mass spectrometry (ESI/MS, LC/MS/MS) and spectrofluorimetric method. Obtained results suggest that metformin dose-dependently inhibits early stage of Maillard reaction, although with the weaker potency than known glycation inhibitor aminoguanidine.
MeSH terms
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Chromatography, Liquid / methods
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Diabetes Mellitus / drug therapy
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Dietary Proteins / metabolism*
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Dose-Response Relationship, Drug
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Glycation End Products, Advanced / metabolism*
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Glycosylation / drug effects
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Humans
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Hypoglycemic Agents / pharmacology*
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Maillard Reaction / drug effects*
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Mass Spectrometry / methods
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Metformin / pharmacology*
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Myoglobin / drug effects
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Serum Albumin / drug effects*
Substances
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Dietary Proteins
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Glycation End Products, Advanced
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Hypoglycemic Agents
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Myoglobin
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Serum Albumin
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Metformin