Isolation of genetic material from Arabidopsis seeds

Methods Mol Biol. 2011:773:151-64. doi: 10.1007/978-1-61779-231-1_10.

Abstract

Here, we describe a series of methods suitable for the reproducible and abundant isolation of total RNA, genomic DNA, and total protein from dry or imbibed Arabidopsis seeds. The resulting material is suitable for most standard molecular biology procedures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics*
  • DNA, Plant / genetics
  • DNA, Plant / isolation & purification*
  • Plant Proteins / genetics
  • Plant Proteins / isolation & purification*
  • RNA, Plant / genetics
  • RNA, Plant / isolation & purification*
  • Seeds / chemistry
  • Seeds / genetics

Substances

  • DNA, Plant
  • Plant Proteins
  • RNA, Plant