Abstract
A yeast-Escherichia coli shuttle vector containing the M13 origin of replication has been constructed. This vector allows selection and replication in both Saccharomyces cerevisiae and E. coli, as well as single-stranded packaging from E. coli upon infection with a helper phage. The presence of a polylinker with various unique restriction sites facilitates the cloning of desired genes.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Cloning, Molecular
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Coliphages / genetics*
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DNA Replication*
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Escherichia coli / genetics*
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Escherichia coli / growth & development
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Genetic Vectors*
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Mitosis
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Molecular Sequence Data
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Plasmids*
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Restriction Mapping
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / genetics*
Associated data
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GENBANK/M55338
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GENBANK/M55339