Objective: To study the supermolecular interaction between different concentrations of hydroxypropyl-beta-cyclodextrin (HP-beta-CD) and imperatorin (IM) and isoimperatorin (ISO) using phase solubility analysis.
Methods: Different concentrations of hydroxypropyl-beta-cyclodextrin were prepared, added overdose of imperatorin or isoimperatorin, made a phase solubility diagram.
Results: The relationship of phase solubility was approximatively linear and the model of inclusion compound was AL and the inclusion ratio of IM, ISO versus HP-beta-CD was 1: 1. The inclusion constants were 214.4, 587.2 L/mol respectively.
Conclusions: Supermolecular inclusion of HP-beta-CD of imperatorin/isoimperatorin could evidently increase the solubility of imperatorin and isoimperatorin, the enhancing effect of isoimperatorin solubility is better than that of imperatorin.