Ultra-selective and sensitive DNA detection by a universal apurinic/apyrimidinic probe-based endonuclease IV signal amplification system

Xianjin Xiao; Chen Song; Chen Zhang; Xin Su; Meiping Zhao

doi:10.1039/c1cc15360a

Ultra-selective and sensitive DNA detection by a universal apurinic/apyrimidinic probe-based endonuclease IV signal amplification system

Chem Commun (Camb). 2012 Feb 14;48(14):1964-6. doi: 10.1039/c1cc15360a. Epub 2011 Oct 25.

Authors

Xianjin Xiao¹, Chen Song, Chen Zhang, Xin Su, Meiping Zhao

Affiliation

¹ Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

PMID: 22027843
DOI: 10.1039/c1cc15360a

Abstract

A novel signal amplification system that is applicable to any DNA sequence of interest has been developed by using a combination of apurinic/apyrimidinic probe and endonuclease IV. The system allows rapid, highly selective and sensitive detection of target DNA sequences at very low concentrations (10 fmol) or low abundance levels (1%).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA / analysis*
DNA Probes / chemistry*
Deoxyribonuclease IV (Phage T4-Induced) / metabolism*
Rhodamines / chemistry
Spectrometry, Fluorescence*

Substances

5-carboxytetramethylrhodamine succinimidyl ester
DNA Probes
Rhodamines
DNA
Deoxyribonuclease IV (Phage T4-Induced)