DNAzyme-based fluorescent microarray for highly selective and sensitive detection of lead(II)

Analyst. 2012 Jan 7;137(1):70-2. doi: 10.1039/c1an15633k. Epub 2011 Oct 28.

Abstract

A facile microarray-based fluorescent sensor for the detection of lead (II) was developed based on the catalytic cleavages of the substrates by a DNAzyme upon its binding to Pb(2+). The release of the fluorophore labelled substrates resulted in the decrease of fluorescence intensity. The sensor had a quantifiable detection range from 1 nM to 1 μM and a selectivity of >20 fold for Pb(2+) over other metal ions.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / methods*
  • Catalytic Domain
  • Cations, Divalent
  • DNA, Catalytic / chemistry
  • DNA, Catalytic / metabolism*
  • Fluorescent Dyes
  • Lead / analysis*
  • Lead / chemistry
  • Lead / metabolism
  • Microarray Analysis / methods*
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence / methods*

Substances

  • Cations, Divalent
  • DNA, Catalytic
  • Fluorescent Dyes
  • Lead