Inactivation of MYO5B promotes invasion and motility in gastric cancer cells

Dig Dis Sci. 2012 May;57(5):1247-52. doi: 10.1007/s10620-011-1989-z. Epub 2011 Dec 2.

Abstract

Background: Loss of cell polarity and tissue disorganisation are hallmarks of cancer. MYO5B mutations disrupt epithelial cell polarity, suggesting that MYO5B may be involved in tumorigenesis.

Methods: We analyzed MYO5B expression in 70 gastric cancer tissues by immunohistochemistry using a tissue microarray method. Two related proteins, Rab11a and TfR, were also investigated.

Results: We found that the negative rate of MYO5B was 78.6 and 17.1% in gastric cancer and normal gastric tissues (P < 0.001), respectively. The MYO5B expression had a strong relationship with Rab11a expression (P = 0.002). We also found that inactivation by siRNA against MYO5B promoted the proliferation, invasion and migration of gastric cancer cells.

Conclusion: The expression of MYO5B was downregulated in gastric cancer and inactivation of MYO5B may contribute to tumorigenesis. Therefore, MYO5B may become an important biomarker for gastric cancer in the future.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / metabolism
  • Cell Line, Tumor
  • Cell Movement
  • Cell Polarity*
  • Cell Transformation, Neoplastic / genetics
  • Cell Transformation, Neoplastic / metabolism
  • Down-Regulation
  • Epithelial Cells* / metabolism
  • Epithelial Cells* / pathology
  • Female
  • Gene Expression
  • Humans
  • Immunohistochemistry
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • Mutation
  • Myosin Heavy Chains* / genetics
  • Myosin Heavy Chains* / metabolism
  • Myosin Type V* / genetics
  • Myosin Type V* / metabolism
  • Neoplasm Grading
  • Neoplasm Invasiveness / genetics*
  • Neoplasm Staging
  • RNA, Small Interfering / metabolism
  • Receptors, Transferrin / metabolism
  • Stomach / pathology
  • Stomach Neoplasms* / genetics
  • Stomach Neoplasms* / metabolism
  • Stomach Neoplasms* / pathology
  • rab GTP-Binding Proteins / metabolism

Substances

  • Antigens, CD
  • CD71 antigen
  • MYO5B protein, human
  • RNA, Small Interfering
  • Receptors, Transferrin
  • Myosin Type V
  • rab11 protein
  • Myosin Heavy Chains
  • rab GTP-Binding Proteins